西寶生物提供廣泛用于各類酶底物、分子生物學、轉基因、人造AMC-肽酶底物、蛋白質的膜分離/分子生物學、ELISA檢測/免疫學、食品微生物學、環境微生物學、植物生長激素等原料試劑，歡迎垂詢！

一. PLANT MOLECULAR BIOLOGY 植物分子生物學

轉基因煙草、樹苗，在camv-35s推動下表達gus(β-葡（萄）糖苷酶)。這些樹苗在37℃培養，加入1mg/ml的X-glcUA, MagentaTM-glcUA,或SalmonTM-glcUA時，可以保持性能。

Gus reporter system:
Transgenic tobacco seedlings, eXpressing gus (beta-Glucuronidase) under the control of the CaMV-35S promoter.The seedlinqs were stained using standard conditions with vacuum infiltration of either X-glcUA, MagentaTM-glcUA, or SalmonTM-glcUA at l mg per ml, with overnight incubation at 37℃.

products:
Enzyme substrates: （各類酶底物）
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D-glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LaposTM -β-D-glcUA·CHX 5-Bromo-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D-glcUA·CHX 6-Bromo-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6100 YTM -β-D-glcUA·CHX 3-Indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D-glcUA·Na 3-Indoxyl-beta-D-glucuronic acid, sodium salt
M-5700 MUG, 4-MU-β-D-glcUA 4-Methylumbelliferyl- beta-D-glucuronic acid
N-4450 PNP-β-D-glcUA 4-Nitrophenyl- beta-D-glucuronic acid
N-4465 PNP-β-D-glcUA·Na 4-Nitrophenyl- beta-D-glucuronic acid, sodium salt
P-2480 Phenolphthalein-β-D-glcUA Phenolphthalein- beta-D-glucuronic acid
P-4150 Phenyl-β-D-glcUA Phenyl- beta-D-glucuronic acid

Plant growth regulators: （植物生長激素）

G-2700 G A3 Gibberellic acid
I-1000 IAA Indole-3-acetic acid
I-2000 IBA Indole-3-butyric acid
K-4000 Kinetin
Z-3000 Trans-Zetain Riboside

二. ALPHA COMPLEMENTATION/MOLECULAR BIOLOGY 分子生物學：

Iacz基因作為一種通訊基因在微生物分子生物學中被頻繁使用，Iacz編譯一種能夠水解底物X-β-D-gal，形成一種靛藍染料沉淀，很多常見的無性繁殖的帶菌者在DNA片斷插入復制時都有Iacz基因被表達DNA片斷插入Iacz片斷時，通常阻礙Iacz片斷工作，只有一小部分Iacz基因片斷用于保持質粒體大小。Iacz基因超過3000個堿基，而且大部分復制質粒體比它還小，這些小片斷叫作α區，位于Iacz基因起始端，α區基因可以通過基因互補來控制β葡萄糖苷酶的活性，所以細胞中Iacz基因被檢測出α區變異。β葡萄糖苷酶的α片斷和形成活性β葡萄糖苷酶的變異的α片斷相第影響，那此被無性繁殖的帶菌都插入的細菌可以被真實的檢出。藍色菌落表示α互補，白色或無色就可能是有克隆片斷插入Iacz基因。
IPTG通過阻斷Iac增強了β-葡（萄）糖苷酶的活性。
培養基和生長條件：LB瓊脂平板，加入100mg/ml X-β-D-gal， 100mg/ml IPTG 37℃培養24小時。

LacZ reporter gene system
The lacZ gene is frequently used in molecular biology as a reporter gene. LacZ encodes the enzyme beta-Galactosidase which can hydrolyze the substrate X-β-D-gal (5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside, B-7150) to form a precipitating blue indigo dye. Many popular cloninq vectors have part of the lacZ gene to indicate when DNA fragments are cloned (inserted) into them. The DNA fragment, tLen inserted into the lacZ fragment, usually inactivates (blocks) the lacZ gene fragment from working. Qnly a Small fragment of the lacZ gene is used in order to keep the size of the plasmid small. (The IacZ gene is over 3000 base pairs long, and most cloninq plasmids are smaller than that.) This small fragment is called the alpha region and is located in the beginning ot the lacZ gene. The alpha portion of the gene can result in beta-Galactosidase enzyme activity by a process called “intragenic complemen-tation” whereby the rest of the lacZ gene is provided in the cell by an alpha mutant of the lacZ gene.The alpha fragment of the beta-Galactosidase enzyme interacts with the complementary portion of the enzyme provided by the alpha mutant to form an active beta-Galactosidase enzyme. Therefore,bacterial colonies can be visually screened to determine which colonies have the cloning vector inserted. Blue colonies show alpha complemen-tation, While white or colorless colonies probably have the cloned fragment inserted within the lacZ gene.
IPTG (Isopropyl-beta-D-thiogalactopyranoside, I-8000) enhances beta-Galactosildase activity by blocking the lac repressor. (by David Demirjian and Tom Jung)
Culture media and growth conditions
Luria-Bertani (LB) agar plates with 100 μg per ml of X-β-D-gal (5-Bromo-4-chloro-3-indoxyl-beta-D- galactopyranoside, B-7150), 100μg per ml of lPTG (lsopropyl-beta-D-thiogalactopyranoside, I-8000). Plates were incubated for 24 hours at 37℃. (Courtesy of Dr. Lawrence Restaino)

products:
Substrates for beta-Galactosidase:
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
B-7200 MagentaTM -β-D-gal 5-Bromo-6-chloro-3-indoxyl-beta-D-galactopyranoside
B-8900 LapisTM -β-D-gal 5-Bromo-3-indoxyl-beta-D-galactopyranoside
C-5000 SalmonTM -β-D-gal 6-Chloro-3- indoxyl-beta-D-galactopyranoside
F-5500 F-β-D-gal 6-Fluoro-3- indoxyl-beta-D-galactopyranoside
I-6000 YTM -β-D-gal 3-Indoxyl- beta-D-galactopyranoside
I-7600 Iodo-β-D-gal 5-Iodo-3- indoxyl- beta-D-galactopyranoside
M-4100 GreenTM -β-D-gal N-Methylindoxyl- beta-D-galactopyranoside
Inducer:
I-8000 IPTG Isopropyl- beta-D-galactopyranoside

被Luc因子表達的熒光素酶的特殊細胞將表現出熒光。
The presence of Iuciferase expressed by the Iuc gene is indicated by a glow or flash emitted form the particular well as illustrated above.
SEEBIO’S products:
L-8200 D-Luciferin Firefly, free acid (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D-Luciferin Firefly, sodium salt (synth.)

清潔劑/表面活性劑
以下的表面活性劑被廣泛的應用于生化分離提純和化驗，這些表面活性劑在離析和提純膜蛋白和其他分子蛋白時是特別有用的。

Detergents/Surtactants
The following detergents can be used in a variety of biochemical separations, purifications, and assays. These surfactants are especially useful in the isolation and purification of membrane proteins and ther cellular proteins.
C-5910 Cholic acid, sodium salt
D-0280 n-Decyl-beta-D-glucopyranoside
D-0290 n-Decyl-4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside
D-3100 Digitonin UDP24
D-3200 Digitonin special grade (Water soluble)
D-8820 n-Dodecyl-beta-D-glucopyranoside
D-8823 n-Dodecyl-beta-D-maltoside
D-8824 n-Dodecyl-d25-beta-D-maltoside
E-8900 Ethyl-beta-D-thiogalactopyranoside
H-1750 n-Heptyl-beta-D-glucopyranoside
H-1758 n-Heptyl-beta-D-thioglucopyranoside
H-1770 n-Hexyl-beta-D-glucopyranoside
N-8400 n-Nonyl-beta-D- glucopyranoside
O-2000 n-Octyl- beta-D- glucopyranoside
O-2700 n-Octyl- beta-D- thiogalactopyranoside
O-2710 n-Octyl-beta-D-thioglucopyranoside
S-6500 Sodium taurocholate
S-6560 Sodium taurodeoxychloate
S-6600 Sodium tauroglycocholate
Numerous permutations o different sugars, thiosugars, and chain lengths are possible.
Please inquire for your custom made detergents/surfactants.

五. ELISA-TESTING/IMMUNOLOGY ELISA檢測/免疫學

提供很多種酶聯免疫檢測的重要底物，可溶的、可溶顯色的、熒光的一樣非常重要的底物。

免疫學
Zymosan A是一種不可溶的酵母的細胞壁，主要有蛋白質，碳水化合物組合而成，能夠用于阻止補足物的活動。
SEEBIO是最大的Zymosan A的生產商，生產很多公斤級原料，每一批都檢測其生物活性。

其他的Zymosans 請詢價

SEEBIO offers a number of important substrates which are useful in various types of ELISA tests. Soluble, chromogenic substrates as well as soluble, fluoroscent substrates are available.
SEEBIO’s products:
Horseradish Peroxidase:（辣根過氧化物酶）
D-2000 HDCBS·Na 3,5-Dichloro-2-hydroxybenzenesulfonic acid, sodium salt
D-2050 HDCBS·diNa 3,5-Dichloro-2-hydroxybenzenesulfonic acid, disodium salt
T-2100 TMB 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 TMB·diHCl 3,3’,5,5’-Tetramethylbenzidine dihydrochloride
Soluble substrates for Alkaline Phosphatase:（堿性磷酸酶的可溶性底物）
M-5800 4-MU-phos 4-Methylumbelliferyl phosphate, free acid
M-550 4-MU-phos·diAMPD 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanediol) salt
M-5900 4-MU-phos·diCHX 4-Methylumbelliferyl phosphate, dicyclohexylammonium salt
M-5950 4-MU-phos·diNa 4-Methylumbelliferyl phosphate, disodium salt
N-4700 PNP- phos·diCHX 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 PNP- phos·diNa 4-Nitrophenyl phosphate, disodium salt

Zymosan A
Zymosan A (Z-9500) is an insoluble cell wall of yeast mainly consisting of protein carbohydrate complexes wich can be used to inactivate complements. (Prepared according to the procedure of Pillemer, et. al., J. Exp. Med. 103:1 (1956).)
SEEBIO Is the largest manufacturer of Zymosan A using proprietary technology producing multi-kilogram lots of the material. Each lot is tested for its biological actilvity.
Please inquire also for other Zymosans.

E. coli O157:H7（病原性大腸桿菌）是一種危險的損傷E. coli 時常存在于漢堡，雞肉和其他肉食品中。根據USDA大概10000人每年因為不熟的包含著E. coli O157:H7的肉制品而染病，這個危險的有機體能夠引起很多疾病甚至死亡。
培養基和生長條件：
麥克拉肯山梨（糖）醇 瓊脂平盤含100ug/ml X-glcUA·Na(BClG), 35℃過液，大部分的含β-葡（萄）糖苷酸酶的E. coli會和酶底物X-glcUA·Na反應成為藍色菌落，沒有β-葡（萄）糖苷酸酶的大腸桿菌為無色狀態。

Testing for E. coli O157:H7
E. coli O157:H7 is a dangerous strain of E.coli which is sometimes present in hamburger, chicken, and other meat products, According to the USDA, as many as 10,000 people each year become sick from eating undercooked meat containing E. coli O157:H7. This dangerous organism can cause severe illness and even death.
Culture media and growth conditions
MacConkey Sorbitol Agar Plates with 100 micrograms per ml of X-glcUA·Na (also known as BClG). Plates were incubated overnight at 35℃. Most E. coli contain the enzyme beta-Glucuronidase, which reacts with the enzyme substrate X-glcUA·Na to produce the deep blue seen in the colonies. E. coli O157:H7 lacks the beta-Glucuronidase enZyme, and the colonies remain basically colorless. (Courtesy of Dr. Lawrence Restaino)

SEEBIO’s products:
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D- glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LapisTM -β-D- glcUA·CHX 5-Bromo-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D- glcUA·CHX 6-Chloro-3- indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
I-6000 YTM -β-D- glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D- glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt
M-5700 MUG,4-MU -β-D-glcUA 4-Methylumbelliferyl- beta-D- glucuronic acid

BCM? O 157:H7(+)
l Plates stable for over 60 days
l False negatives are raw
l More specitic than other plating medium
l Isolates over 2 times more E. coli O 157:H7 than standard USDA plating medium
These data are confirmed by independent investigations and through governmental evaluations as well as publications in USA, Europe and Japan
SEEBIO’s product:
C-0500 BCM? O 157:H7(+) Plating Medium
Please ask for more information, including the protocol and literature.
平板保持穩定60天以上
自然陰性狀態
比其它平板培養基更多的規格
比標準USDA底盤培養基多2次分離E. coli O 157:H7（病原性大腸桿菌）
這些數據是通過和美國，歐洲，日本之類的政府的評價和獨立的調查得出的結論

BCM? Listeria monocytogenes Detection System
l The best available test system with a fluorogenic selective enrichment broth and a chromogenic plating medium
l Confirmation within 6 to 24 hours after isolation
l The pre-enrichment is selective and repairs injured cells
l 2.5 times more Listeria monocytogenes isolated than the conventional USDA method
l The selective enrichment broth is fluorogenic and thus can be applied in an automated equipment
l Potential false positives are eliminated sequentially by the system’s selectivity

SEEBIO’s product:
C-0600 BCM? Listeria monocytogenes Detection System
Please ask for more information, including the protocol and literature
BCM? 單包李斯特菌的探測體系：
一種最可行的用熒光選擇探測的體系，金色平板
分離后6-24小時內可確認
選擇性的預濃縮并且修復受損細胞
比常規的USDA方法多分離2.5倍多的單包李斯特菌
選擇性的濃縮肉湯含熒光的，也可用于自動化操作
人造的（正電壓）陽離子被這個體系選擇性的持續的消除

BCM? Bacillus cereus and Bacillus thuringiensis
l A new fascinating test isolating both important organisms within 24 hours
l Other Bacillus species either form white colonies or do not grow
l There is no equivalent test on the market

SEEBIO’s product:
C-0700 BCM? Bacillus cereus and Bacillus thuringiensis
Please ask for more information, including the protocol and literature.
BCM? 蠟狀芽孢桿菌和蘇云金芽孢桿菌
一種新的可以在24小時內分離重要生物體的神奇的方法
其他桿菌種類為白色菌落或不生長
在市場上沒有同類產品（方法）

ATP化驗 ATP和蟲熒光素就是螢火蟲當閃動的時候有細菌存在就有ATP存在。在蟲熒光酶催化下，ATP和螢火蟲的熒光素反應，發出熒光。熒光的大小和樣品中ATP含量成正比，熒光的測驗非常的敏感，因此可以快速準確得出樣品是否被細菌污染。
ATP occurs wherever bacteria are present. Reaction of ATP with Luciferin Firetly in the presence of Luciferase results in the emission of light as a glow or flash. The amount of light emitted is directly proportional to the amoumt of ATP present in the sample. The measurement of the emitted light is very sensitive thus allowing quick and accurate conclusions of the bacterial contamination of the sample.

SEEBIO’s product:
L-8200 D-Luciferin Firefly, free acid (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D-Luciferin Firefly, sodium salt (synth.)
L-8080 Luciferase, American Firefly, natural

水和土壤的測試
SEEBIO為微生物鑒定提供一系列的酶底物環境篩選測試，包括β-葡萄糖苷酶和水中、土壤樣本中細菌的β-葡萄糖苷酶的檢測。

Water & Soils Testing
SEEBIO offers a wide range of enzyme substrates for the identification of microorganisms. Environ-mental screening tests most often involve the detection of beta-Glucuronidase and beta-Galactosidase positive bacteria in water and soil samples.
SEEBIO’s product:
Substrates for beta-Galactosidase:
A-7050 p-Aminophenyl-β-D-gal 4-Aminophenyl-beta-D-galactopyranoside
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D- galactopyranoside
B-7200 MagentaTM -β-D- gal 5-Bromo-6-chloro-3-indoxyl-beta-D- galactopyranoside
B-8900 LapisTM -β-D- gal 5-Bromo-3-indoxyl- beta-D- galactopyranoside
C-5000 SalmonTM -β-D- gal 6-Chloro-3- indoxyl- beta-D- galactopyranoside
F-5500 F-β-D- gal 6-Fluoro-3- indoxyl- beta-D- galactopyranoside
I-6000 YTM -β-D- gal 3-Indoxyl- beta-D- galactopyranoside
I-7600 Iodo-β-D- gal 5-Iodo-3- indoxyl- beta-D- galactopyranoside
M-4100 GreenTM -β-D- gal N-Methylindoxyl- beta-D- galactopyranoside
M-5550 4-MU -β-D-gal 4-Methylumbelliferyl- beta-D- galactopyranoside
N-4190 ONP-β-D- gal 2-Nitrophenyl- beta-D- galactopyranoside
N-4300 NPN-β-D- gal 4- Nitrophenyl- beta-D- galactopyranoside
P-4100 Phenyl -β-D-gal Phenyl- beta-D- galactopyranoside

Enhancer for beta-Galactosidase:
I-8000 IPTG Isopropyl-beta-D-thiogalactopyranoside

Substrates for beta-Galactosidase:
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7350 MagentaTM -β-D- glcUA·CHX 5-Bromo-6-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 LapisTM -β-D- glcUA·CHX 5-Bromo-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 SalmonTM -β-D- glcUA·CHX 6-Chloro-3- indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
I-6100 YTM -β-D- glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexylammonium salt
I-6140 YTM -β-D- glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt
M-5700 MUG,4-MU -β-D-glcUA 4-Methylumbelliferyl- beta-D- glucuronic acid
N-4450 PNP-β-D-glcUA 4-Nitrophenyl- beta-D- glucuronic acid
N-4465 PNP-β-D-glcUA·Na 4-Nitrophenyl- beta-D- glucuronic acid, sodium salt
P-2480 Phenolphthalein-β-D-glcUA Phenolphthalein - beta-D- glucuronic acid
P-4150 Phenyl -β-D- glcUA Phenyl- beta-D- glucuronic acid

Enhancer for beta-Glucuronide:
M-3600 O-Me-glcUA·Na 1-O-Methyl- beta-D- glucuronic acid, sodium salt
I-8350 IPTGA Isopropyl - beta-D- thioglucuronic acid

AMC底物在特定酶反應時會發出熒光信號，這些底物是非常敏感的，而且他們非常適合自動化測試。
SEEBIO有高級的所有的實際合成新科技的發展，AMC派生物是必須的，因此，SEEBIO可以人工制造提取您所需要的AMC底物。
SEEBIO已經發展新的技術生產合成任何被需要的AMC衍生物，因此，SEEBIO也可以按您的特殊要求訂做AMＣ底物。

Custom made AMC-Peptidase Substrates
AMC substrates (7-Amido-4-methylcoumarin substrates) give a soluble, fluorescent signal upon reaction with the correct enzyme. These substrates are extremely sensitive and are well extremely sensitive and are well suited to tests performed in automated instrumentation or robotics.
SEEBIO has developed proprietary new technology for synthesizing virtually any AMC derivative that might be required. Therefore, SEEBIO can custom manufacture the exact AMC substrate needed in your particular application.

SEEBIO’s standard AMC products:
A-1470 Ac-Lys-AMC·AcOH N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 H-D-Ala-AMC·TFA D-Alanine 7- amido-4-methylcoumarin, trifluoroacetate salt
A-4500 H-Ala-AMC·TFA L- Alanine 7- amido-4-methylcoumarin, trifluoroacetate salt
A-8700 H-Arg-AMC·diHCl L-Arginine 7- amido-4-methylcoumarin dihydrochloride
A-8750 H-Arg-Arg-AMC·HCl L-Arginyl-L-arginine 7- amido-4-methylcoumarin hydrochloride
A-9300 H-Asp-AMC L-Aspartic acid alpha-(7- amido-4-methylcoumarin)
A-9305 H-Asp-AMC·TFA L-Aspartic acid alpha-(7- amido-4-methylcoumarin), trifluoroacetate salt
C-2100 N-α-CBZ- Arg-AMC·HCl N-alpha-CBZ-L-Arginine 7- amido-4-methylcoumarin hydrochloride
C-2128 CBZ-Lys-Lys- Arg-AMC·triAcOH N-CBZ-Lysyl-lysyl-arginine 7- amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Gly-Pro-AMC N-CBZ-Glycyl-L-proline 7- amido-4-methylcoumarin
C-6200 H-Cit- AMC·HBr L-Citrulline 7- amido-4-methylcoumarin hydrochloride
C-6300 H-Cit- AMC·TFA L-Citrulline 7- amido-4-methylcoumarin, trifluoroacetate salt
G-3845 H-Glu(OH)-AMC L-Glutamic acid alpha-(7- amido-4-methylcoumarin)
G-3850 H-Glu-AMC L-Glutamic acid gamma-(7- amido-4-methylcoumarin)
G-3950 Glutaryl-Gly-Arg-AMC Glutaryl-glycyl-L-arginine (7- amido-4-methylcoumarin)
G-4500 H-Gly- AMC·HBr Glycine 7- amido-4-methylcoumarin hydrochloride
G-6100 H-Gly-Gly-AMC·HCl Glycyl-glycine 7- amido-4-methylcoumarin hydrochloride
G-6300 Gly-Phe-AMC Glycyl-L-phenylalanine 7- amido-4-methylcoumarin
G-6350 Gly-Pro- AMC·HBr Glycyl-L-proline 7- amido-4-methylcoumarin hydrochloride
H-2350 H-His-AMC L-Histidine 7- amido-4-methylcoumarin
H-7300 H-Hyp-AMC L-Hydroxyproline 7- amido-4-methylcoumarin
I-7900 H-lle-AMC·TFA L-Isoleucine 7- amido-4-methylcoumarin, trifluoroacetate salt
L-2750 H-Leu-AMC L-Leucine 7- amido-4-methylcoumarin
L-2800 H-Leu- AMC·HCl L-Leucine 7- amido-4-methylcoumarin hydrochloride
L-9300 H-Lys- AMC·AcOH L-Lysine 7- amido-4-methylcoumarin, acetate salt
M-3170 H-Met- AMC·AcOH L-Methionine 7- amido-4-methylcoumarin, acetate salt
M-3200 H-Met- AMC·TFA L-Me thionine 7- amido-4-methylcoumarin, trifluoroacetate salt
O-3400 H-Orn-AMC·Cab L-Ornithine 7- amido-4-methylcoumarin, carbonate salt
P-3500 H-Phe- AMC·TFA L-Phenylalanine 7- amido-4-methylcoumarin, trifluoroacetate salt
P-7280 H-Pro- AMC·HBr L-Proline 7- amido-4-methylcoumarin hydrochloride
P-8500 H-Pyr-AMC L-Pyroglutamic acid 7- amido-4-methylcoumarin
S-2700 H-Ser- AMC·HCl L-Serine 7- amido-4-methylcoumarin hydrochloride
S-3300 H-Ser-Tyr-AMC L-Seryl-L-tyrosine 7- amido-4-methylcoumarin
T-8350 H-Trp-AMC L-Tryptophan 7- amido-4-methylcoumarin
T-8355 H-Trp- AMC·HCl L-Tryptophan 7- amido-4-methylcoumarin hydrochloride
T-9100 H-Tyr-AMC L-Tyrosine 7- amido-4-methylcoumarin
V-2000 H-Val-AMC L-Valine 7- amido-4-methylcoumarin
V-2100 H-Val- AMC·TFA L-Valine 7- amido-4-methylcoumarin, trifluoroacetate salt

Different salts and N-protected AMC derivatives for pptide synthesis are also available from SEEBIO.
Please inquire for any AMC substrate that may be needed in your application.

酶的誘導劑和增強劑
SEEBIO提供一些既能誘導也能增強若干重要的酶／底物的反應的試劑，我們最新的很有效的誘導劑是Isopropyl-beta-D-thioglucuronic acid，sodiumsalt, Isopropyl-beta-D-thioglucuronic acid，sodiumsalt是一個不參與反應的誘導劑，他不會被酶水解，而且能夠很有效的增強β-葡（萄）糖苷酸酶在大腸桿菌測試中敏感性。

Enzyme inducers and enhancers：
SEEBIO offers a number of reagents which either induce or enhance several important enzyme/substrate reactions. Our most recent and very effective inducer is Isopropyl-beta-D-thioglucuronic acid, sodium salt (I-8350).
Isopropyl-beta-D-thioglucuronic acid sodium salt is a “gratuitous” inducer, i.e. it is not hydrolyzed by the enzyme, and enhances very effectively the sensitivity of your beta-Glucuronidase test in E. coli by increasing the concentration of the enzyme.
The following compounds can also be used in conjumction with SEEBIO substrates to enhance color development:
alpha-Galactosidase M-3590 1-O-Methyl-alpha-D-galactopyranoside
beta-Galactosidase E-8900 Ethyl-beta-D-thiogalactopyranoside
I-8000 Isopropyl-beta-D- thiogalactopyranoside
M-4440 Methyl- beta-D- thiogalactopyranoside
M-3591 1-O-Methyl- beta-D- galactopyranoside
alpha-Glucosidase M-3594 3-O-Methyl-alpha-D-glucopyranoside
beta-Glucosidase M-3592 1-O-Methyl-beta-D- glucopyranoside
beta-Glucuronidase I-8350 Isopropyl-eta-D-thioglucuronic acid, sodium salt
M-3600 1-O-Methylglucuronic acid, sodium salt
alpha-Mannosidase M-4150 1-O-Methyl-alpha-D-mannopyranoside
Alkaline phosphatase N-8100 NBT chloride
T-2300 TNBT chloride
beta-Xylosidase M-6600 1-O-Methyl-beta-D-xylopyranoside
Miscellaneous I-1900 Indole-3-acrylic acid

假單胞銅綠菌中分離
C-390是一種抗菌劑加入到瓊脂培養基是，可以有效地將假單胞銅綠菌從其他細菌分離出來。

使用情況：多種細菌最小抑制濃度實驗的結果表示，C-390在25ug/ul或更少濃度時，抑制微生物（除假單）的生長，然而MICS包含從50到大于100ug/ml的假單胞菌銅綠菌，因此C-390被評價為是在假單胞菌瓊脂F中的最有潛力的選擇性選擇試劑，修復試驗在53株假單胞菌銅綠菌的存在下進行，結果和在其他培養基作對比，顯示假單胞菌瓊脂F含30ug/ml C-390抑制性小而選擇性大，加入C-390的假單胞菌瓊脂F，有選擇性和差異性，大量的證據同樣告訴我們，C-390可以被加入到其他基礎培養基中，得到同樣的結果，文獻著作權屬SEEBIO所有。

C-390, 9-Chloro-9-(4’-diethyamino) phenyl-9,10-dihydro-10-phenylacridine, hydrochloride
(SEEBIO catalog number:C-3900)
Isolation of Pseudomonas aeruginosa
C-390 is an antibacterial agent which is useful for the selective isolation of Pseudononas aeruginosa from other bacterial species when added to an agar medium.
Patent: U.S. 4,263,398(4/21/81)
Use: Results of minimal inhibitory concentration tests with a diversity of bacterial strains showed that C-390 inhibited the growth of all microorganisms tested (other than Pseudononas aeruginosa) at 25μg/μl or less, whereas MICs obtained for Pseudononas aeruginosa ranged from 50 to >100μg/ml. Therefore, C-390 was evaluated as a potential selective agent for Pseudononas aeruginosa in pseudomonas agar F.Recovery tests were conducted on this medium with 53 strains of Pseudononas aeruginosa, and the results were compared to those obtained in similar tests on commercially available selective media, i.e. pseudomonas isolation agar and Psersosel agar. The results of these comparisons indicated that pseubomonas agar F with C-390(30μg/ml) was significantly less inhibitory than Pseudosel agar and pseudomonas isolation agar and more selective than pseudomonas isolation agar. The incorporation of C-390 in pseudomonas agar F also provided a medium that was both selective and differential. Preliminary evidence also suggested that C-390 may be added to other basal media with comparable results. Literature is available from SEEBIO.


GENERAL MICROBIOLOGY
普通微生物學：
顯色酶底物被很廣泛的用于食品微生物學，環境微生物學，臨床微生物學
SEEBIO開發了大量的其他底物，請檢查我們的酶在字母排列表中的以考信息，另外，我們鼓勵您因為其他的您需要的但不在我們目錄中的底物而聯系我們，我們很高興去尋找您所需要的合成的產品的可能性。

Chromogenic enzyme substrates are widely used in
FOOD MICROBIOLOGY
ENVIRONMENTAL MICROBIOLOGY
CLINICAL MICROBIOLOGY
SEEBIO carries a large variety of these and other substrates. Please check out our enzyme cross reference listing and/or our detailed alphabetical listing. Furthermore, we encourage you to contact SEEBIO regarding any substrate that you may need, but we do not list in our catalog. We will be glad to explore the possibilities for custom synthesis of your substrate of choice.


CHROMOGENIC SUBSTRATES
Indoxyl Substrates
e.g. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-gal) Catalog-#B-7150
The same basic reaction applies to the following chromogenic indigo substrate derivatives:
Substrates Color of precipitate Absorption λmax[nm]
X Blue 615
YTM Blue 680
MagentaTM Magenta 565
SalmonTM Salmon 540
Iodo Purple 575
GreenTM green 665

SEEBIO’s product:
BLUE I:
Substrates for Glycosidases:
B-6950 X-β-D-galactosaminide 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
B-7000 X-β-D-glucosaminide 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7110 X-β-D-cellobioside 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside
B-7135 X-α-L-fucoside 5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside
B-7136 X-β-D-fucoside 5-Bromo-4-chloro-3-indoxyl-beta-D- fucopyranoside
B-7138 X-β-L-fucoside 5-Bromo-4-chloro-3-indoxyl-beta-L- fucopyranoside
B-7141 X-α-D-gal 5-Bromo-4-chloro-3-indoxyl-alpha-D-galactopyranoside
B-7150 X-gal, X-β-D-gal 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
B-7230 X-α-D-glc 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucopyranoside
B-7250 X-β-D-glc 5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside
B-7300 X-β-D-glcUA·CHX 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 X-β-D-glcUA·Na 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-7407 X-α-D-maltotrioside 5-Bromo-4-chloro-3-indoxyl-alpha-D-maltotrioside
B-7409 X-α-D-mannoside 5-Bromo-4-chloro-3-indoxyl-alpha-D-mannopyranoside
B-7750 X-β-D-xyl 5-Bromo-4-chloro-3-indoxyl-beta-D-xylopyranoside

Substrates for Esterases:
B-7050 X-butyrate 5-Bromo-4-chloro-3-indoxyl butyrate
B-7100 X-caprylate 5-Bromo-4-chloro-3-indoxyl caprylate
B-7410 X-nonanoate 5-Bromo-4-chloro-3-indoxyl nonanoate
B-7412 X-oleate 5-Bromo-4-chloro-3-indoxyl oleate
B-7414 X-palmitate 5-Bromo-4-chloro-3-indoxyl palmitate

Substrate for Phosphatases:
B-7420 X-phos·diAMPD 5-Bromo-4-chloro-3-indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
B-7450 X-phos·diNa 5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt
B-7500 X-phos·p-tol 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt

Substrate for Sulfatases:
B-7600 X-sulfate·K 5-Bromo-4-chloro-3-indoxyl sulfate, potassium salt


BLUE II:
Substrates for Glycosidases:
I-6000 YTM -β-D- gal 3-Indoxyl- beta-D- galactopyranoside
I-6050 YTM -β-D- glc 3-Indoxyl- beta-D- glucopyranoside
I-6100 YTM -β-D-glcUA·CHX 3-Indoxyl- beta-D- glucuronic acid, cyclohexlammonium salt
I-6140 YTM -β-D-glcUA·Na 3-Indoxyl- beta-D- glucuronic acid, sodium salt

Substrate for Esterases:
I-5680 YTM -butyrate 3-Indoxyl butyrate

Substrates for Phosphatases:
I-6200 YTM - phos·diAMPD 3-Indoxyl- phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
I-6250 YTM - phos·diNa 3-Indoxyl- phosphate, disodium salt
I-6300 YTM - phos·p-tol 3-Indoxyl- phosphate, p-toluidine salt

Substrate for Sulfatases:
I-6350 YTM - sulfate·K 3-Indoxyl- sulfate, potassium salt

MAGENTA:
Substrates for Glycosidases:
B-7020 MagentaTM -β-D-glucosaminide 5-Bromo-6-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7195 MagentaTM -α-D-gal 5-Bromo-6-chloro-3-indoxyl-alpha-D-galactopyranoside
B-7200 MagentaTM -β-D-gal 5-Bromo-6-chloro-3-indoxyl-beta-D- galactopyranoside
B-7270 MagentaTM -β-D-glc 5-Bromo-6-chloro-3-indoxyl- beta-D-glucopyranoside
B-7350 MagentaTM -β-D-glcUA 5-Bromo-6-chloro-3-indoxyl-beta-D- glucuronic acid, cyclohexylammonium salt

Substrates for Esterases:
B-7060 MagentaTM -butyrate 5-Bromo-6-chloro-3-indoxyl-butyrate
B-7095 MagentaTM -caprate 5-Bromo-6-chloro-3-indoxyl-caprate
B-7102 MagentaTM -caprylate 5-Bromo-6-chloro-3-indoxyl-caprylate
B-7411 MagentaTM -nonaoate 5-Bromo-6-chloro-3-indoxyl- nonaoate
B-7416 MagentaTM -palmitate 5-Bromo-6-chloro-3-indoxyl-palmitate

Substrate for Phosphatases:
B-7550 MagentaTM - phos·p-tol 5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt

Substrate for Sulfatases
B-7650 MagentaTM - sulfate·K 5-Bromo-6-chloro-3-indoxyl phosphate, potassium salt

SALMON:
Substrates for Glycosidases:
C-4762 SalmonTM -β-D-galactosaminide 6-chloro-3-indoxyl- N-acetyl-beta-D-galactosaminide
C-4830 SalmonTM -β-D-cellobioside 6-chloro-3-indoxyl- beta-D-cellobioside
C-4995 SalmonTM -α-D-gal 6-chloro-3-indoxyl- alpha-D-galactopyranoside
C-5000 SalmonTM -β-D-gal 6-chloro-3-indoxyl- beta-D- galactopyranoside
C-5020 SalmonTM -β-D-glc 6-chloro-3-indoxyl- beta-D-glucopyranoside
C-5050 SalmonTM -β-D-glcUA·CHX 6-chloro-3-indoxyl- beta-D-glucuronic acid, cyclohexylammonium salt
C-5070 SalmonTM -α-D-mannoside 6-chloro-3-indoxyl- alpha-D-mannopyranoside

Substrates for Esterases:
C-4800 SalmonTM -butyrate 6-chloro-3-indoxyl butyrate
C-4820 SalmonTM -caprylate 6-chloro-3-indoxyl caprylate
C-5075 SalmonTM -nonanoate 6-chloro-3-indoxyl nonanoate
C-5080 SalmonTM -palmitate 6-chloro-3-indoxyl palmitate

Substrates for Phosphatases:
C-5100 SalmonTM -phos·p-tol 6-chloro-3-indoxyl phosphate, p-toluidine salt

PURPLE:
I-7600 Iodo -β-D-gal 5-Iodo-3-indoxyl-beta-D-galactopyranoside

GREEN:
M-4100 GreenTM -β-D-gal N-Methylindoxyl-beta-D-galactopyranoside

Oxidizing Agents:
N-8100 NBT Nitrotetrazolium blue chloride
T-2300 TNBT Tetranitro blue tetrazolium chloride

2-and 4-Nitrophenyl (ONP and PNP) Substrates
e.g. 4-Nitrophenyl phosphate, disodium salt (PNP-phos) Catalog-#:N-5010
The same basic reaction applies for all 2-and 4-Nitrophenyl (ONP and PNP) substrates.

SEEBIO’s products:
Substrates for Glycosidases:
N-3970 PNP-α-D-galactosaminide 2-Nitrophenyl-N-acetyl-alpha-D-galactosaminide
N-3990 PNP-β-D-galactosaminide 4-Nitrophenyl-N-acetyl-beta-D-galactosaminide
N-4014 ONP-α-D-glucosaminide 2-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
N-4022 ONP-β-D-glucosaminide 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide
N-4030 PNP-α-D- glucosaminide 4-Nitrophenyl-N-acetyl-alpha-D- glucosaminide
N-4050 PNP-β-D- glucosaminide 4-Nitrophenyl-N-acetyl- beta -D- glucosaminide
N-4070 PNP-α-L-arabinofuranoside 4-Nitrophenyl-alpha-L-arabinofuranoside
N-4100 PNP-β-D-cellobiose 4-Nitrophenyl – beta-D-cellobioside
N-4130 PNP-α-L-fucoside 4-Nitrophenyl-alpha-L-fucopyranoside
N-4150 PNP-β-D-fucoside 4-Nitrophenyl- beta-D -fucopyranoside
N-4160 PNP-β-L-fucoside 4-Nitrophenyl- beta-L -fucopyranoside
N-4190 ONP-β-D-gal 2-Nitrophenyl-beta-D-galactopyranoside
N-4200 PNP-α- D-gal 4-Nitrophenyl- alpha -D-galactopyranoside
N-4300 PNP-β- D-gal 4- Nitrophenyl-beta -D-galactopyranoside
N-4320 ONP-β-D-glc 2- Nitrophenyl-beta -D-glucopyranoside
N-4330 MNP-β-D-glc 3- Nitrophenyl-beta -D-glucopyranoside
N-4350 PNP-α- D-glc 4-Nitrophenyl- alpha -D- glucopyranoside
N-4400 PNP-β- D-glc 4-Nitrophenyl- beta -D- glucopyranoside
N-4450 PNP-β- D-glcUA 4-Nitrophenyl- beta -D- glucuronic acid
N-4465 PNP-β- D-glcUA·Na 4-Nitrophenyl- beta -D- glucuronic acid, sodium salt
N-4480 PNP-β- D-lac 4-Nitrophenyl- beta -D-lactopyranoside
N-4487 PNP-α- D-maltopentaoside 4-Nitrophenyl-alpha-D-maltopentaoside
N-4490 PNP-α- D-maltoside 4-Nitrophenyl-alpha-D-maltopyranoside
N-4492 PNP-β- D- maltoside 4-Nitrophenyl- beta -D-maltopyranoside
N-4500 PNP-α- D-mannoside 4-Nitrophenyl-alpha-D-mannopyranoside
N-4550 PNP-α- D-mannoside 4-Nitrophenyl-beta-D-mannopyranoside
N-5290 ONP-β- D-xyl 2-Nitrophenyl-beta-D-xylopyranoside
N-5300 PNP-β- D-xyl 4-Nitrophenyl-beta-D-xylopyranoside

Substrate for Esterases:
N-4600 PNP-palmitate 4-Nitrophenyl palmitate

Substrates for Phosphatases:
N-4700 PNP-phos·CHX 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 PNP-phos·diNa 4-Nitrophenyl phosphate, disodium salt
Horseradish Peroxidase/TMB System

SEEBIO’s products:
T-2100 TMB 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 TMB·diHCl 3,3’,5,5’-Tetramethylbenzidine dihydrochloride


十二. FLUOROGENIC SUBSTRATES
Methylumbelliferyl (4-MU-) Substrates
e.g. 4-Methylumbelliferyl-beta-D-glucuronic acid (4-MU-glcUA) Catalog-#: M-5700
The same basic reaction applies for all 4-Methylumbelliferyl substrates:

SEEBIO’s products:
Substrates for Glycosidases:
M-5500 4-MU-β- D- galactosaminide 4-Methylumbelliferyl-N-acetyl-beta-D-galactosaminide
M-5504 4-MU-β- D- glucosaminide 4-Methylumbelliferyl-N-acetyl-beta-D-glucosaminide
M-5510 4-MU-α- L- arabinoside 4-Methylumbelliferyl-alpha-L-arabinopyranoside
M-5530 4-MU-β- D-cellobioside 4-Methylumbelliferyl- beta-D-cellobioside
M-5539 4-MU-α- L-fucoside 4-Methylumbelliferyl-alpha-L-fucopyranoside
M-5540 4-MU-β- D-fucoside 4-Methylumbelliferyl-beta-D-fucopyranoside
M-5541 4-MU-β- L-fucoside 4-Methylumbelliferyl-beta-L-fucopyranoside
M-5544 4-MU-α-D-gal 4-Methylumbelliferyl-alpha-D-galactopyranoside
M-5550 4-MU-β-D-gal 4-Methylumbelliferyl-beta-D-galactopyranoside
M-5600 4-MU-α-D-glc 4-Methylumbelliferyl-alpha-D-glucopyranoside
M-5650 4-MU-β-D-glc 4-Methylumbelliferyl-beta-D-glucopyranoside
M-5700 MUG, 4-MU-β-D-glcUA 4-Methylumbelliferyl-beta-D-glucuronic acid
M-5718 4-MU-β-D-lactoside 4-Methylumbelliferyl-beta-D-lactoside
M-5730 4-MU-α-D-mannoside 4-Methylumbelliferyl-alpha-D-mannopyranoside
M-5738 4-MU-β-D-mannoside 4-Methylumbelliferyl-beta-D-mannopyranoside
M-6500 4-MU-β-D-xyl 4-Methylumbelliferyl-beta-D-xylopyranoside

Substrates for Esterases:
M-5520 4-MU-butyrate 4-Methylumbelliferyl butyrate
M-5526 4-MU-caprylate 4-Methylumbelliferyl caprylatd
M-5744 4-MU-nonanoate 4-Methylumbelliferyl nonanoate
M-5750 4-MU-palmitate 4-Methylumbelliferyl palmitate

Substrates for Phosphatases:
B-3500 2(4-MU)-phos Bis(4-methylumbelliferyl) phosphate
M-5800 4-MU-phos 4-Methylumbelliferyl phosphate, free acid
M-5850 4-MU-phos·diAMPD 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
M-5900 4-MU-phos·diCHX 4-Methylumbelliferyl phosphate, dicyclohexylammonium
M-5920 4-MU-phos·diLi 4-Methylumbelliferyl phosphate, dilithium salt
M-5950 4-MU-phos·diNa 4-Methylumbelliferyl phosphate, disodium salt trihydrate

Substrate for Sulfatases:
M-6200 4-MU-sulfate·K 4-Methylumbelliferylsulfate, potassium salt


十三. 7-Amido-4-methylcoumarin (AMC) Substrates
e.g. Lysine 7-Amido-4-methylcoumarin Catalog-#: L-9300
The same basic reaction applies for all 7-Amido-4-methylcoumarin (AMC) substrates.

SEEBIO’s products:
A-1470 Ac-Lys-AMC·AcOH N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 H-D-Ala- AMC·TFA D-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-4500 H-Ala- AMC·TFA L- Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-8700 H-Arg- AMC·diHCl L-Arginine 7-amido-4-methylcoumarin dihydrochloride
A-8750 H- Arg-Arg- AMC·HCl L-Arginyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
A-9300 H-Asp-AMC L-Aspartic acid alpha- (7-amido-4-methylcoumarin)
A-9305 H-Asp-AMC·TFA L-Aspartic aicd alpha- (7-amido-4-methylcoumarin), rtifluoroacetate salt
C-2100 H-α-CBZ-Arg- AMC·HCl N-alpha-CBZ-L-Arginie (7-amido-4-methylcoumarin) hydrochloride
C-2128 CBZ-Lys-Lys-Arg- AMC·triAcOH N-CBZ-Lysyl-lysyl-arginine 7-amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Gly-Pro-AMC N-CBZ-Glycyl-L-proline 7-amido-4-methylcoumarin
C-6200 H-Cit- AMC·HBr L-Citrulline 7-amido-4-methylcoumarin hydrobromide
C-6300 H- Cit- AMC·TFA L-Citrulline 7-amido-4-methylcoumarin, trifluoroacetate salt
G-3845 H-Glu(OH)-AMC L-Glutamic acid alpha-(7-amido-4-methylcoumarin)
G-3850 H-Glu-AMC L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3950 Glutaryl-Gly-Arg-AMC Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin
G-4500 H-Gly- AMC·HBr Glycine 7-amido-4-methylcoumarin hydrobromide
G-6100 H-Gly-Gly-AMC·HCl Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride
G-6300 Gly-Phe-AMC Glycyl-L-phenylalanine 7-amido-4-methylcoumarin
G-6350 Gly-Pro- AMC·HBr Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide
H-2350 H-His-AMC L-Histidine 7-amido-4-methylcoumarin
H-7300 H-Hyp-AMC L-Hydroxyproline 7-amido-4-methylcoumarin
I-7900 H-lle- AMC·TFA L-Isoleucine 7-amido-4-methylcoumarin, trifluoroacetate salt
L-2750 H-Leu-AMC L-Leucine 7-amido-4-methylcoumarin
L-2800 H-Leu-AMC·HCl L-Leucine 7-amido-4-methylcoumarin hydrochloride
L-9300 H-Lys- AMC·AcOH L-Lysine 7-amido-4-methylcoumarin, acetate salt
M-3170 H-Met- AMC·AcOH L-Methionine 7-amido-4-methylcoumarin, acetate salt
M-3200 H- Met- AMC·TFA L-Methionine 7-amido-4-methylcoumarin, trifluoroacetate salt
O-3400 H-Orn-AMC·Cab L-Ornithine 7-amido-4-methylcoumarin, carbonate salt
P-3500 H-Phe- AMC·TFA L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt
P-7280 H-Pro- AMC·HBr L-Proline 7-amido-4-methylcoumarin hydrobromide
P-8500 H-Pyr-AMC L-Pyroglutamic acid 7-amido-4-methylcoumarin
S-2700 H-Ser- AMC·HCl L-Serine 7-amido-4-methylcoumarin hydrochloride
S-3300 H-Ser-Tyr-AMC L-Seryl-L-tyrosine 7-amido-4-methylcoumarin
T-8350 H-Trp-AMC L-Tryptophan 7-amido-4-methylcoumarin
T-8355 H-Trp- AMC·HCl L- Tryptophan 7-amido-4-methylcoumarin hydrochloride
T-9100 H-Tyr-AMC L-Tyrosine 7-amido-4-methylcoumarin
V-2000 H-Val-AMC L-Valine 7-amido-4-methylcoumarin
V-2100 H-Val- AMC·TFA L- Valine 7-amido-4-methylcoumarin, trifluoroacetate salt
Different salts and N-protected AMC derivatives for peptide synthesis are also available from BIOSYNH.
Please inquire for any AMC substrate that may be needed in your application.

BIOLUMINESCENT SUBSTRATES
Firefly Luciferase Substrates
e.g. Luciferin Firefly, free acid, synth. Catalog-#: L-8200
The same basic reaction occurs when Luciferase is added to the system or Luciferase is expressed by a reporter gene.
SEEBIO’s products:
L-8200 D-Luciferin Firefly (synth.)
L-8220 D-Luciferin Firefly, potassium salt (synth.)
L-8240 D- Luciferin Firefly, sodium slat (synth.)
L-8080 Luciferase, American Firetly, natural

Apoaequorin-and Renilla Luciferase-Substrate: Coelenterazine catalog-#: C-7000
The different wavelengths compared to applications with Firefly Luciferase make double reporter gene applications possible.
SEEBIO’s products:
C-7000 Coelenterazine
D-0400 2-(4-Dehydroxy) coelenterazine
Glycosidases:
alpha-Amylase B-7407 5-Bromo-4-Chloro-3-indoxyl-alpha-D-maltotrioside
N-4487 4-Nitrophenyl-alpha-D-maltopentaoside
alpha-L-Arabinosidase M-5510 4-Methylumbelliferyl-alpha-L-arabinopyranoside
N-4070 4-Nitrophenyl-alpha-L-arabinofuranoside
beta-Cellobiosidase B-7110 5- Bromo-4-Chloro-3-indoxyl-beta-D-cellobioside
C-4830 6-Chloro-3-indoxyl-beta-D-cellobioside
M-5530 4-Methylumbelliferyl-beta-D- cellobioside
N-4100 4-Nitrophenyl- beta-D- cellobioside
alpha-L-Fucosidase B-7135 5-Bromo-4-Chloro-3-indoxyl-alpha-L-fucopyranoside
N-2230 2-Naphthyl-alpha-L- fucopyranoside
M-5539 4-Methylumbelliferyl- alpha-L- fucopyranoside
N-4130 4-Nitrophenyl- alpha-L- fucopyranoside
beta-D-Fucosidase B-7136 5-Bromo-4-Chloro-3-indoxyl-beta-D-fucopyranoside
M-5540 4-Methylumbelliferyl-beta-D- fucopyranoside
N-4150 4-Nitrophenyl-beta-D- fucopyranoside
beta-L-Fucosidase B-7138 5-Bromo-4-Chloro-3-indoxyl-beta-L-fucopyranoside
M-5541 4-Methylumbelliferyl-beta-L- fucopyranoside
N-4160 4-Nitrophenyl-beta-L- fucopyranoside
alpha-Galactosaminidase N-3970 2-Nitrophenyl-N-acetyl-alpha-D-galactosaminide
beta- Galactosaminidase B-6950 5-Bromo-4-Chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
C-4762 6-Chloro-3-indoxyl-N-acetyl-beta-D-galactosaminide
M-5500 4-Methylumbelliferyl-N-acetyl-beta-D-galactosaminide
N-3990 4-Nitrophenyl-N-acetyl-beta- D-galactosaminide
alpha-Galactosidase B-7141 5-Bromo-4-Chloro-3-indoxyl-alpha-D-galactopyranoside
B-7195 5-Bromo-4-Chloro-3-indoxyl-alpha-D-galactopyranoside
C-4995 6-Chloro-3-indoxyl-alpha-D-galactopyranoside
M-5544 4-Methylumbelliferyl-alpha-D-galactopyranoside
N-2250 1-Naphthyl-alpha-D-galactopyranoside
N-4200 4-Nitrophenyl-alpha-D-galactopyranoside
beta- Galactosidase A-7050 4-Aminophenyl-beta-D- galactopyranoside
B-7150 5-Bromo-4-Chloro-3-indoxyl-beta-D-galactopyranoside
B-7200 5-Bromo-6-Chloro-3-indoxyl-beta-D-galactopyranoside
B-8900 5-Bromo-3-indoxyl-beta-D-galactopyranoside
C-5000 6-Chloro-3-indoxyl-beta-D-galactopyranoside
F-5500 6-Fluoro-3-indoxyl-beta-D-galactopyranoside
H-7500 8-Hydroxyquinoline- beta-D-galactopyranoside
I-6000 3-Indoxyl-beta-D-galactopyranoside
I-7600 5-Iodo-3-indoxyl-beta-D-galactopyranoside
M-4100 N-Methylindoxyl-beta-D-galactopyranoside
M-5550 4-Methylumbelliferyl-beta-D-galactopyranoside
N-2030 Naphthol AS-BI-beta-D-galactopyranoside
N-2260 2-Naphthyl- beta-D-galactopyranoside
N-4190 2-Nitrophenyl-beta-D-galactopyranoside
N-4300 4-Nitrophenyl-beta-D-galactopyranoside
P-4100 Phenyl-beta-D-galactopyranoside
alpha-Glucosaminidase N-4014 2-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
N-4030 4-Nitrophenyl-N-acetyl-alpha-D-glucosaminide
beta-Glucosaminidase B-7000 5-Bromo-4-Chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
B-7020 5-Bromo-6-Chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide
M-5504 4-Methylumbelliferyl-N-acetyl-beta-D-glucosaminide
N-4022 2-Nitrophenyl-N-acetyl-beta-D-glucosaminide
N-4050 4-Nitrophenyl-N-acetyl-beta-D-glucosaminide
alpha-Glucosidase B-7230 5-Bromo-4-Chloro-3-indoxyl-alpha-D-glucopyranoside
M-5600 4-Methylumbelliferyl-alpha-D-glucopyranoside
N-2300 2-Naphthyl-alpha-D-glucopyranoside
N-4350 4-Nitrophenyl-alpha-D-glucopyranoside
beta-Glucosidase B-7250 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucopyranoside
B-7270 5-Bromo-6-Chloro-3-indoxyl-beta-D-glucopyranoside
C-5020 6-Chloro-3-indoxyl-beta-D-glucopyranoside
I-6050 3-Indoxyl-beta-D-glucopyranoside
M-5650 4-Methylumbelliferyl-beta-D-glucopyranoside
N-2210 1-Naphthyl-N-acetyl-glucosaminide
N-2290 1-Naphthyl-beta-D-glucopyranoside
N-4320 2-Nitrophenyl-beta-D-glucopyranoside
N-4330 3-Nitrophenyl-beta-D-glucopyranoside
N-4400 4-Nitrophenyl-beta-D-glucopyranoside
P-4100 Phenyl-beta-D-galactopyranoside
P-4130 Phenyl-beta-D-glucopyranoside
beta-Glucuronidase B-7300 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylamonium salt
B-7350 5-Bromo-6-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
B-7400 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt
B-8902 5-Bromo-4-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
C-5050 6-Chloro-3-indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
H-7600 8-Hydroxyquinoline-beta-D-glucuronic acid
I-6100 3-Indoxyl-beta-D-glucuronic acid, cyclohexylammonium salt
I-6140 3-Indoxyl-beta-D-glucuronic acid, sodium salt
M-5700 4-Methylumbelliferyl-beta-D-glucuronic acid
N-4450 4-Nitrophenyl-beta-D-glucuronic acid
N-4465 4-Nitrophenyl-beta-D-glucuronic acid, sodium salt
P-4150 Phenyl-beta-D-glucuronic acid
beta-Lactosidase M-5718 4-Methylumbelliferyl-beta-D-lactoside
N-4480 4-Nitrophenyl-beta-D-lactopyranoside
alpha-Maltosidase N-4490 4-Nitrophenyl-alpha-D-maltopyranoside
alpha-Mannosidase B-7409 5-Bromo-4-Chloro-3-indoxyl-alpha-D-mannopyranoside
B-8930 2-(6-Bromonaphthyl)-alpha-D-mannopyranoside
C-5070 6-Chloro-3-indoxyl-alpha-D-mannopyranoside
M-5730 4-Methylumbelliferyl-alpha-D-mannopyranoside
N-4500 4-Nitrophenyl-alpha-D-mannopyranoside
beta-Mannosidase M-5738 4-Methylumbelliferyl-beta-D-mannopyranoside
N-4550 4-Nitrophenyl-beta-D-mannopyranoside
beta-Xylosidase B-7750 5-Bromo-4-Chloro-3-indoxyl-beta-D-xylopyranoside
B-8940 2-(6-Bromonaphthyl)-beta-D-xylopyranoside
M-6500 4-Methylumbelliferyl-beta-D-xylopyranoside
N-5290 2-Nitrophenyl-beta-D-xylopyranoside
N-5300 4-Nitrophenyl-beta-D-xylopyranoside
Esterases:
Carboxyl Esterase B-7050 5-Bromo-4-Chloro-3-indoxyl butyrate
B-7100 5-Bromo-4-Chloro-3-indoxyl caprylate
Cholinesterase B-9600 S-Butyrythiocholine iodide
Esterase(misc.) B-6244 5-Bromo-4-Chloro-3-indoxyl-3-acetate
B-6304 5-Bromo-6-Chloro-3-indoxyl-3-acetate
B-7050 5-Bromo-4-Chloro-3-indoxyl butyrate
B-7060 5-Bromo-6-Chloro-3-indoxyl butyrate
B-7095 5-Bromo-6-Chloro-3-indoxyl caprate
B-7100 5-Bromo-4-Chloro-3-indoxyl caprylate
B-7102 5-Bromo-6-Chloro-3-indoxyl caprylate
B-7140 5-Bromo-4-Chloro-3-indoxyl nonanoate
B-7411 5-Bromo-6-Chloro-3-indoxyl nonanoate
B-7412 5-Bromo-4-Chloro-3-indoxyl oleate
B-7414 5-Bromo-4-Chloro-3-indoxyl palmitate
B-7416 5-Bromo-6-Chloro-3-indoxyl palmitate
C-4758 6-Chloro-3-indoxyl-3-acetate
C-4800 6-Chloro-3-indoxyl butyrate
C-4820 6-Chloro-3-indoxyl caprylate
C-5075 6-Chloro-3-indoxyl nonanoate
C5080 6-Chloro-3-indoxyl palmitate
I-5600 3-Indoxyl-3-acetate
I-5680 3-Indoxyl butyrate
M-5520 4-Methylumbelliferyl butyrate
M-5526 4-Methylumbelliferyl caprylate
M-5744 4-Methylumbelliferyl nonanoate
M-5750 4-Methylumbelliferyl palmitate
N-4600 4-Nitrophenyl palmitate
Phosphatases:
Alkaline Phosphatase B-7450 5-Bromo-4-Chloro-3-indoxyl phosphate, disodium salt
B-7500 5-Bromo-4-Chloro-3-indoxyl phosphate, p-toluidine salt
B-7550 5-Bromo-6-Chloro-3-indoxyl phosphate, p-toluidine salt
C-5100 6-Chloro-3-indoxyl phosphate, p-toluidine salt
I-6200 3-Indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
I-6250 3-Indoxyl phosphate, disodium salt
I-6300 3-Indoxyl phosphate, p-toluidine salt
M-5800 4-Methylumbelliferyl phosphate, free acid
M-5850 4-Methylumbelliferyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
M-5900 4-Methylumbelliferyl phosphate, dicyclohexylammonium salt
M-5920 4-Methylumbelliferyl phosphate, dilithium salt
M-5950 4-Methylumbelliferyl phosphate, disodium salt trihydrate
Phosphatase(misc.) B-3500 Bis (4-methylumbelliferyl) phosphate
B-7420 5-Bromo-4-Chloro-3-indoxyl phosphate, di(2-amino-2-methyl-1,3-propanedio) salt
N-2370 1-Naphthyl phosphate, calcium salt
N-2400 1-Naphthyl phosphate, sodium salt
N-2450 2-Naphthyl phosphate, monosodium salt
N-4700 4-Nitrophenyl phosphate, dicyclohexylammonium salt
N-5000 4-Nitrophenyl phosphate, disodium salt
P-2360 Phenolphthalein diphosphate, tetrasodium salt
Peptidases:
Cathepsin A-8700 L-Arginine 7-amido-4-methylcoumarin dihydrochloride
Dipeptidyl Aminopeptidase II L-9400 L-Lysyl-L-alanine-4-methoxy-beta-naphthylamide hydrobromide
Gamma-Glutamyl Transpeptidase G-3850 L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3920 L-gamma-Glutamyl-4-nitroanilide monohydrate
Pyroglutamyl Peptidase P-8500 L-Pyroglutamic acid 7-amido-4-methylcoumarin
P-8600 L-Pyroglutamic acid-2-naphthylamide
Peptidase(misc.) A-1470 N-Acetyl-L-lysine 7-amido-4-methylcoumarin, acetate salt
A-4220 D-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-4500 L-Alanine 7-amido-4-methylcoumarin, trifluoroacetate salt
A-8700 L-Arginine 7-amido-4-methylcoumarin hydrochloride
A-8750 L-Arginyl-L-arginine 7-amido-4-methylcoumarin dihydrochloride
A-9300 L-Aspartic acid alpha-(7-amido-4-methylcoumarin)
A-9305 L-Aspartic acid alpha-(7-amido-4-methylcoumarin), trifluoroacetate salt
C-2100 N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride
C-2128 N-CBZ-Lysyl-lysyl-arginine 7-amido-4-methylcoumarin, triacetate salt
C-2150 N-CBZ-Glycyl-L-proline 7-amido-4-methylcoumarin
C-6200 L-Citrulline 7-amido-4-methylcoumarin hydrobromide
C-6300 L-Citrulline 7-amido-4-methylcoumarin, trifluoroacetate salt
G-3845 L-Glutamic acid alpha-(7-amido-4-methylcoumarin)
G-3850 L-Glutamic acid gamma-(7-amido-4-methylcoumarin)
G-3950 Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride
G-4500 Glycine 7-amido-4-methylcoumarin hydrobromide
G-6100 Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride
G-6300 Glycyl-L-phenylalanine 7-amido-4-methylcoumarin
G-6350 Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide
H-2350 L-Histidine 7-amido-4-methylcoumarin
H-7300 L-Hydroxyproline 7-amido-4-methylcoumarin hydrochloride
I-7900 L-Isoleucine 7-amido-4-methylcoumarin, trifluoroacetate salt
L-2750 L-Leucine 7-amido-4-methylcoumarin
L-2800 L-Leucine 7-amido-4-methylcoumarin hydrochloride
L-9300 L-Lysine 7-amido-4-methylcoumarin, acetate salt
M-3170 L-Methionine 7-amido-4-methylcoumarin, acetate salt
M-3200 L-Methionine 7-amido-4-methylcoumarin, trifluoroacetate salt
O-3400 L-Ornithine 7-amido-4-methylcoumarin, carbonate salt
P-3500 L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt
P-7280 L-Proline 7-amido-4-methylcoumarin hydrobromide
P-8500 L-Pyroglutamic acid 7-amido-4-methylcoumarin
S-2700 L-Serine 7-amido-4-methylcoumarin hydrochloride
S-3300 L-Seryl-L-tyrosine 7-amido-4-methylcoumarin
T-8350 L-Tryptophan 7-amido-4-methylcoumarin
T-8355 L-Tryptophan 7-amido-4-methylcoumarin hydrochloride
T-9100 L-Tyrosine 7-amido-4-methylcoumarin
V-2000 L-Valine 7-amido-4-methylcoumarin
V-2100 L-Valine 7-amido-4-methylcoumarin trifluoroacetate salt

Sulfatases:
Arylsulfatase B-7600 5-Bromo-4-Chloro-3-indoxyl sulfate, potassium salt
B-7650 5-Bromo-6-Chloro-3-indoxyl sulfate, potassium salt
I-6350 3-Indoxyl sulfate, potassium salt
M-6200 4-Methylumbelliferyl sulfate, potassium salt
Sulfatases(misc.) P-2400 Phenolphthalein disulfate, tripotassium salt

Miscellaneous Enzymes:
N-AcetylneuraminicAcid Aldotase A-1600 N-Acetylneuraminic acid
Horseradish Peroxidase T-2100 3,3’,5,5’-Tetramethylbenzidine, free base
T-2150 3,3’,5,5’-Tetramethylbenzidine dihydrochloride
Luciferase L-8200 D-Luciferin Firely (synth.)
L-8220 D-Luciferin Firely, potassium salt (synth.)
L-8240 D-Luciferin Firely, sodium salt (synth.)
Phosphatidylinositol Phospholipase C(PI-PLC) B-7404 5-Bromo-4-Chloro-3-indoxyl-myo-inositol-1-phosphate, ammonium salt
N-4474 4-Nitrophenyl-myo-inositol-1-yl phosphate, ammonium salt
Phosphodiesterase I B-3700 Bis (4-nitrophenyl) phosphate, sodium salt
Phosphodiesterase II B-7680 5-Bromo-4-Chloro-3-indoxyl thymidine-3’-phosphate, cyclohexylammonium salt
Urokinase G-3950 Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride

Enzyme Inducers & Enhancers:
alpha-Galactosidase M-3590 1-O-Methyl-alpha-D-galactopyranoside
beta-Galactosidase E-8900 Ethyl-beta-D-thiogalactopyranoside
M-3591 1-O-Methyl-beta-D-galactopyranoside
M-4440 Methyl-beta-D-thiogalactopyranoside
I-8000 Isopropyl-beta-D-thiogalactopyranoside
alpha-Glucosidase M-3594 3-O-Methyl-alpha-D-glucopyranose
beta-Glucosidase I-8200 Isopropyl-beta-D-thioglucopyranoside
M-3592 1-O-Methyl-beta-D-glucopyranoside
M-4450 Methyl-beta-D-thioglucopyranoside
beta-Glucuronidase I-8350 Isopropyl-beta-D-thioglucuronic aicd, sodium salt
M-3600 1-O-Methyl-beta-D-glucuronic acid, sodium salt
alpha-Mannosidase M-4150 1-O-Methyl-alpha-D-mannopyranoside
Alkaline phosphatase N-8100 Nitrotetrazolium blue chloride
T-2300 Tetranitro blue tetrazolium chloride
beta-Xylosidase M-6600 1-O-Methyl-beta-D-Xylopyranoside
Miscellaneous I-1900 Indole-3-acrylci acid

Cultrue Media Additives:
A-1100 N-Acetyl-L-cysteine
A-1170 N-Acetyl-D-galactosaminide
A-1200 N-Acetyl-D-glucosaminide
A-1600 N-Acetylneuraminic acid
A-3000 Adonitol
A-6100 5-Aminolevulinic acid hydrochloride
A-6150 5-Aminolevulinic aicd methyl ester hydrochloride
C-2600 D-Cellobiose
C-3900 C-390
C-5910 Cholic acid, sodium salt
D-0500 2-Deoxy-D-galactose
D-0520 2-Deoxy-D-glucose
D-0560 2-Deoxy-D-ribose
D-9500 Dulcitol
F-6300 Fluoroorotic aicd
G-1500 D-Galactosamine hydrochloride
H-1700 Hemin
M-0850 L-Malic aicd
M-1500 D-Melezitose
M-1520 D-Melezitose hydrate
M-1700 D-Melibiose monohydrate
N-2525 Neutral Red
R-1000 D-Raffinose pentahydrate
R-3000 L-Rhamnose
R-4000 Resazurin, sodium salt
R-5500 D-Ribose
S-1500 D-Salicin
S-5500 Sodium polyanetholesulfonate
S-6000 Sodium pyruvate
S-6500 Sodium taurocholate
T-3600 Thiomerosal
T-5000 D-Trehalose dihydrate
Z-3000 trans-Zeatin Riboside

Detergents
C-5910 Cholic acid, sodium salt
D-0280 n-Decyl-beta-D-glucopyranoside
D-0290 n-Decyl-4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside
D-3100 Digitonin USP24
D-3200 Digitonin special grade (water soluble)
D-8820 n-Dodecyl-beta-D-glucopyranoside
D-8823 n-Dodecyl-beta-D-maltoside
D-8824 n-Docecyl-d25-beta-D-maltoside
E-8900 Ethyl-beta-D-thiogalactopyranoside
H-1750 n-Heptyl-beta-D-glucopyranoside
H-1758 n-Heptyl-beta-D-thioglucopyranoside
H-1770 n-Hexyl-beta-D-glucopyranoside
N-8400 n-Nonyl-beta-D-glucopyranoside
O-2000 n-Octyl-beta-D-glucopyranoside
O-2700 n-Octyl-beta-D-thiogalactopyranoside
O-2710 n-Octyl-beta-D-thioglucopyranoside
S-6500 Sodium taurocholate
S-6560 Sodium taurodeoxychloate
S-6600 Sodium tauroglycocholate

Plant growth regulators
G-2700 Gibberellic acid GA3
I-1000 Indole-3-acetic acid
I-2000 Indole-3-butyric acid
K-4000 Kinetin
Z-3000 Trans-Zeatin Riboside

Oxidizing agents
N-8100 Nitrotetrazolium blue chloride
T-2300 Tetranitro blue tetrazolium chloride

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