D-葡萄糖醛酸/D-半乳糖醛酸檢測試劑盒，D-Glucuronic/D-Galacturonic Acid Assay Kit, 貨號：K-URONIC

來源：作者：人氣：-發表時間：2014-11-07 15:26:00【大中小】

D-葡萄糖醛酸/D-半乳糖醛酸檢測試劑盒產品 貨號：K-URONIC

品牌：Megazyme
產品貨號：K-URONIC
品牌：Megazyme
產品品名：D-葡萄糖醛酸/D-半乳糖醛酸檢測試劑盒
英文品名：D-Glucuronic/D-Galacturonic Assay Kit
規格型號：100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)

A simple, reliable and accurate method for the measurement of D-hexuronic acids (specifically D-glucuronic acid and D-galacturonicacid) in plant extracts, culture media/supernatants and other materials.Suitable for manual, auto-analyser and microplate formats.

INTRODUCTION:

D-glucuronic acid and D-galacturonic acid are naturally occurring hexuronic acids present in glycosaminoglucans, glucuronide conjugates
in mammals and in plant cell wall polysaccharides.D-Glucuronic acid exists as a component of glycosaminoglucans such as hyaluronan, heparin and chondroitin sulphate present in mammalian connective tissue such as cartilage. In the synthesis of glucuronide conjugates in mammals glucuronic acid is conjugated to xenobiotic compounds, a biosynthesis reaction known as glucuronidation, catalysed by the enzyme UDP-glucuronyltransferase and considered a detoxifying process. Both D-glucuronic acid and D-galacturonic acid are major components of plant cell wall polysaccharides. D-glucuronic acid is a component of arabinoxylan, which consists of a β-(1,4)-linked xylan backbone substituted with α-(1-2/3)-linked L-arabinofuranose and α-(1-2)-linked 4-O-methylglucuronic acid. D-Galacturonic acid is the major component of pectin comprising the α-(1,4)-linked galacturonan backbone of homogalacturonan and rhamnogalacturonan II, and is present within the repeating disaccharide unit [,4)-α-D-GalpA-(1,2)-α-L-Rhap-(1,] of rhamnogalacturonan I.This kit (K-URONIC) is suitable for the specific measurement of
D-hexuronic acids including D-glucuronic and D-galacturonic acid.

KITS:

Kits suitable for performing 100 assays in manual format (or 1000 assays in auto-analyser format or 1000 assays in microplate format) are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (22 mL, pH 8) plus sodium azide (0.02% w/v) as a preservative.Stable for > 2 years at 4°C.
Bottle 2: NAD+ freeze dried powder.Stable for > 5 years at -20°C.
Bottle 3: Uronate dehydrogenase suspension (2.2 mL).Stable for > 2 years at 4°C.
Bottle 4: D-Glucuronic acid (5 mL; 0.5 mg/mL).Stable for > 2 years at 4°C.