LBIS(R) 小鼠卵清蛋白特異性免疫球蛋白E（OVA-IgE）ELISA試劑盒

來源：作者：人氣：-發表時間：2024-12-26 09:47:00【大中小】

LBIS® 小鼠卵清蛋白特異性免疫球蛋白E（OVA-IgE） ELISA試劑盒

LBIS® OVA-IgE Mouse

IgE（Immunoglobulin E，免疫球蛋白E）是第五個被發現的免疫球蛋白，由5個結構域（VH、CHε 1～4）構成的兩個Hε鏈和兩個L鏈中組成的IgE，其分子量約190000，電泳實驗中向γ1域移動。IgE的代謝半衰期約3天，正常人血清中的IgE濃度非常低，約300 ng/mL。但在寄生蟲感染和枯草熱時濃度會升高。與過敏性相關的IgE被稱作反應素。因接觸過敏原導致反應素含量升高，反應素會在Fc域與存在于皮膚、呼吸道、消化臟器中嗜堿性粒細胞和肥大細胞的FcεR1受體結合，引起細胞過敏。在結合過敏原后細胞發生脫粒現象，組胺，五羥色胺，蛋白酶，肝素，趨化因子，前列腺素，白三烯等被投放，經過支氣管收縮和黏膜水腫，分泌亢進，從而誘發支氣管哮喘，部分蕁麻疹，過敏性鼻炎，過敏性反應等I型過敏性反應。

本試劑盒是以OVA（卵清蛋白）作為免疫的抗原，在一個簡化的反應系統中通過測定鼠抗OVA- IgE抗體值來進行小鼠免疫系統檢測的試劑盒。

◆特點

●　測定時間短（總反應時間：1小時50分鐘）。

●　微量樣本即可測定。

●　使用無害的防腐劑。

●　全部試劑為溶液即用類型。

●　高測定精度和高重復性。

●　操作簡便，無需進行特殊前處理。

試劑盒組成

組成品	狀態	包裝
OVA包被96孔板（干燥板）	清洗后使用	96 wells（8×12）／1個
標準溶液（Anti OVA-IgE:1, 200 U/mL）（單抗）	稀釋后使用	100 μL/1瓶
緩沖液	直接使用	60 mL/1瓶
生物素結合抗小鼠IgE抗體（單抗）	稀釋后使用	200 μL/1瓶
過氧化物酶·抗生素結合物	稀釋后使用	200 μL/1瓶
顯色液（TMB）	直接使用	12 mL/1瓶
終止液（1M H₂SO₄）※小心輕放	直接使用	12 mL/1瓶
濃縮清洗液（10×）	稀釋后使用	100 mL/1瓶
孔板密封膜		3個
產品說明書		1本

◆樣本信息

●　小鼠血清·血漿

●　10 μL/well（稀釋樣本）

※　樣本需要用試劑盒附帶的緩沖液調至標準曲線范圍內。

※　樣本必須稀釋10倍以上。

◆測定范圍

1.88～120 U/mL（標準曲線范圍）

（本試劑盒中1 U/mL定義為抗原結合常數（Ka）為2.0×10⁸ M-1的抗體1.3 ng/mL）

◆實驗數據

精度測試（組內變異）

樣本	A	B
1	70.7	19.1
2	71.0	18.7
3	77.1	19.6
4	74.3	19.7
5	72.9	18.9
Mean	73.2	19.2
SD	2.6	0.42
CV（%）	3.6	2.2

單位：U/mL

重復性測試（組間變異）

測定日/樣本	C	D	E
0天	60.0	15.0	3.75
1天	59.1	15.0	3.75
2天	58.1	14.7	3.65
3天	63.6	16.0	3.48
Mean	60.2	15.2	3.66
SD	2.4	0.57	0.13
CV（%）	4.0	3.7	3.4

單位：U/mL

加標回收測試

樣本F

添加量	實測值	回收量	回收率（%）
0.00	6.93	—	—
5.35	12.2	5.27	98.5
10.7	17.8	10.9	102
17.1	25.1	18.2	106

單位：U/mL，n=3

樣本G

添加量	實測值	回收量	回收率（%）
0.00	40.5	—	—
30.8	70.4	29.5	95.8
35.9	77.1	36.6	102
53.9	94.9	54.4	101

單位：U/mL，n=3

稀釋直線性測試

2個血清樣本連續用稀釋緩沖液稀釋3個梯度測定結果，直線回歸值R²=0.9987~0.9999

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產品列表

產品編號	產品名稱	產品規格
639-07651	（AKRIE-030）小鼠卵清蛋白特異性免疫球蛋白E(OVA-IgE) ELISA試劑盒 LBIS^® OVA-IgE Mouse	96 tests