β-半乳糖苷酶能夠水解通過(guò)β-1→4糖苷鍵連接在單糖、寡糖或糖肽上的半乳糖殘基。它主要用于酶聯(lián)免疫檢測(cè)中,偶聯(lián)在抗體上作為標(biāo)記物。此外,β-半乳糖苷酶還可用于糖類結(jié)構(gòu)分析、乳糖檢測(cè)和鈉離子檢測(cè)。
Appearance: White powder, lyophilized
Source: E.coli
Enzyme Commission Number: EC 3.2.1.23
CAS Number: 9031-11-2
Storage temperature: -20℃
Specific activity: ≥ 400U/mg protein
Unit definition: One unit will cause the formation of one micromole of ONP from ONPG per min at pH 7.3 at 37℃.
PROPERTIES
Molecular weight: 118 kDa (SDS-PAGE)
Isoelectric point: 4.6
Optimum pH: 7.0
Optimum temperature: 50℃
pH Stability: 5.5~9.5 (25℃,20hr)
Thermal stability: < 45℃ (pH 7.3, 15min)