D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit , 貨號：K-FRUGL
品牌：Megazyme
貨號：K-GLUGL
中文品名：D-果糖/D-葡萄糖檢測試劑盒
品名：D-Fructose plus D-glucose assay kit
規格：110 次檢測 (手工) / 1100 (微孔板) / 1100 (自動分析儀)
應用：D-果糖/D-葡萄糖檢測試劑盒是一種酶法-紫外檢測方法，用于測量和檢測植物和食品中D-果糖,和/或D-葡萄糖含量。輔助因子更穩定，溶解后在4°C下可保存超過1年。適合手工、微孔板和自動分析儀分析。
原理：
檢測方法：分光光度法 @340 nm
反應時間：~13min
檢測限：0.66 mg/L
應用案例：
葡萄酒、啤酒、果汁、軟飲料、牛奶、果醬、蜂蜜、食療食品、面包、焙烘食品、糖果、甜點、糕點、冰淇淋、水果蔬菜、調味品、煙草、化妝品、藥品、造紙和其他原料(如生物培養、樣品等)
方法識別：
基于上述原理的檢測方法已經被AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK和IOCCC等組織接受。
試劑盒組成：
| Bottle 1: | 緩沖溶液(15 mL, pH 7.5) 添加疊氮鈉(0.02%)作為防腐劑 |
| 4°C下穩定超過 2年 | |
| Bottle 2: | NADP+, ATP和PVP。 |
| -20°C穩定超過 5年 | |
| Bottle 3: | 己糖激酶和葡萄糖-6-磷酸脫氫酶懸浮液, 2.25mL. |
| 4°C下穩定超過 2年 | |
| Bottle 4: | 磷酸葡萄糖異構酶懸浮液(2.25 mL)。 |
| 4°C下穩定超過 2年 | |
| Bottle 5: | D-葡萄糖和D-果糖標準溶液(5 mL, 每種糖濃度為0.2 mg/mL)。 |
| 4°C下穩定超過 2年 |
優點：
包含PVP可預防單寧抑制
經過法國Suze la Rousse葡萄酒大學驗證
價格非常具有競爭力
所有試劑制備后可以穩定保存超過2年（針對手工分析應用）
在25°C或37°C下都能快速檢測 官網提供Mega-Calc™ 軟件工具用于一站式原始數據處理
包含標準溶液
輔助因子更穩定
適合手工、微孔板和自動分析儀分析。
經過法國Suze la Rousse葡萄酒大學驗證
價格非常具有競爭力
所有試劑制備后可以穩定保存超過2年（針對手工分析應用）
在25°C或37°C下都能快速檢測 官網提供Mega-Calc™ 軟件工具用于一站式原始數據處理
包含標準溶液
輔助因子更穩定
適合手工、微孔板和自動分析儀分析。
參考文獻：
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Lactose fermentation by Kombucha – a process to obtain new milk–based beverages. Ili?i?, M., Kanuri?, K., Milanovi?, S., Lon?ar, E., Djuri?, M. & Malbaša, R. (2012). Romanian Biotechnological Letters, 17(1), 7013-7021.
Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance. Maioli, M., Pes, G. M., Sanna, M., Cherchi, S., Dettori, M., Manca, E. & Farris, G. A. (2008). Acta Diabetologica, 45(2), 91-96.
Endocrine and metabolic effects of consuming fructose- and glucose-sweetened beverages with meals in obese men and women: Influence of insulin resistance on plasma triglyceride responses. Teff, K. L., Grudziak, J., Townsend, R. R., Dunn, T. N., Grant, R. W., Adams, S. H., Keim, N. L., Cummings, B. P., Stanhope, K. L. & Havel, P. J. (2009). Journal of Clinical Endocrinology & Metabolism, 94(5), 1562-1569.
Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques. Adams, S. H., Stanhope, K. L., Grant, R. W., Cummings, B. P. & Havel, P. J. (2008). Endocrinology, 149(6), 3002-3008.
β-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3. Kasperowicz, A., Stan-Glasek, K., Guczynska, W., Pristas, P., Javorsky, P., Vandzurova, A. & Michalowski, T. (2012). World Journal of Microbiology and Biotechnology, 28(3), 1271-1279.
Comparative effects of fructose and glucose on lipogenic gene expression and intermediary metabolism in HepG2 liver cells. Hirahatake, K. M., Meissen, J. K., Fiehn, O. & Adams, S. H. (2011). PloS one, 6(11), e26583.
Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry. Wahjudi, P. N., Patterson, M. E., Lim, S., Yee, J. K., Mao, C. S. & Lee, W. N. P. (2010). Clinical Biochemistry, 43(1-2), 198-207.
Physiological studies of Leuconostoc mesenteroides strain NRRL B-1149 during cultivation on glucose and fructose media. Bivolarski, V., Vasileva, T., Shukla, R., Goyal, A. & Iliev, I. (2012). Journal of BioScience & Biotechnology, 1(3), 235-240.
Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells. Fu, J., Tay, S. S. W., Ling, E. A. & Dheen, S. T. (2007). Journal of Neurochemistry, 103(4), 1654-1665.
Immobilised yeast grape must deacidification in a recycle fixed bed reactor. Portugal, I., Ribeiro, S. C., Xavier, A. M. R. B., Centeno, F. & Strehaiano, P. (2011). International Journal of Food Science & Technology, 46(2), 284-289.
The Elemental Composition of Synechocystis sp. PCC6803 under Nutrient Limiting Conditions. Blom, M. (2014). University of Amsterdam, Bachelor Thesis.
使用手冊：
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