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可吸收碳水化合物/膳食纖維檢測試劑盒

貨號(hào)：K-ACHDF
包裝：1 Kit

英文名：Available Carbohydrates/Dietary Fiber Assay Kit
級(jí)別：100 assay
品牌：Megazyme

可吸收碳水化合物/膳食纖維檢測試劑盒, Available Carbohydrates/Dietary Fiber Assay Kit, 貨號(hào)：K-ACHDF，用于測量和分析谷物產(chǎn)品，水果、蔬菜和食品中可吸收碳水化合物和膳食纖維的含量。

*西寶提示：我司所銷售的化學(xué)試劑、原料等所有產(chǎn)品（包括但不限于抗生素類、蛋白質(zhì)類、試劑盒類產(chǎn)品等）僅限用于科學(xué)研究用途，不得作用于人體。

訂購熱線：400-021-8158

產(chǎn)品詳情質(zhì)檢信息

可吸收碳水化合物/膳食纖維檢測試劑盒, Available Carbohydrates/Dietary Fiber Assay Kit, 貨號(hào)：K-ACHDF

品牌：Megazyme

貨號(hào)：K-ACHDF

中文品名：可吸收碳水化合物/膳食纖維檢測試劑盒

品名：Available Carbohydrates/Dietary Fiber Assay Kit

規(guī)格：100次

應(yīng)用：用于測量和分析谷物產(chǎn)品，水果、蔬菜和食品中可吸收碳水化合物和膳食纖維的含量。

原理：（膳食纖維）

(α-amylase + amyloglucosidase)

(1) Starch + H2O → D-glucose

(protease)

(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol precipitation

原理：（可吸收碳水化合物）

(sucrase / maltase + β-galactosidase)

(1) Sucrose, maltose and lactose → D-glucose + D-fructose + D-galactose

(PGI, hexokinase + glucose-6-phosphate dehydrogenase)

(2) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate + NADPH + ADP + H+

方法識(shí)別：

膳食纖維 - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
AACC (Methods 32-05.01, 32-07.01 and 32-21.01)

應(yīng)用案例：

食品配料、食品成品和其它食材。

產(chǎn)品列表：

貨號(hào)	品名	規(guī)格
K-ACHDF	Available Carbohydrates/Dietary Fiber Assay Kit	100 assays

試劑盒組成：

100次可吸收碳水化合物/膳食纖維檢測試劑盒 (貨號(hào) K-ACHDF)

Bottle 1:	緩沖溶液 (11 mL, pH 7.6) + 疊氮鈉 (0.02% w/v) 作為防腐劑. 4°C下可穩(wěn)定3年以上
Bottle 2:	NADP+ 加 ATP -20°C可穩(wěn)定5年以上
Bottle 3:	熱穩(wěn)定的α-淀粉酶 (6 mL, 3,000 U/mL) (Megazyme貨號(hào) E-BLAAM) 4°C下可穩(wěn)定3年以上
Bottle 4:	蛋白酶(10 mL, ~ 350 U/mL) (Megazyme貨號(hào) E-BSPRT) -20°C可穩(wěn)定3年以上
Bottle 5:	淀粉葡萄糖苷酶 (20 mL, ~ 3,300 U/mL) (Megazyme貨號(hào) E-AMGDF) 4°C下可穩(wěn)定3年以上
Bottle 6:	己糖激酶加葡萄糖-6-磷酸脫氫酶懸浮液, 2.25 mL 4°C下可穩(wěn)定3年以上
Bottle 7:	磷酸葡萄糖異構(gòu)酶懸浮液 (2.25 mL) Stable for > 3 years at 4°C
Bottle 8:	D-葡萄糖 + D-果糖標(biāo)準(zhǔn)溶液(每種糖5 mL, 0.2 mg/mL) 4°C下可穩(wěn)定3年以上

優(yōu)點(diǎn)：

價(jià)格極具競爭力
所有試劑可穩(wěn)定保存2年以上
僅提供試劑盒
使用高純度標(biāo)準(zhǔn)化的酶
官網(wǎng)上提供Mega-Calc™軟件工具用于有序的原始數(shù)據(jù)處理
形式簡單

參考文獻(xiàn)：

Measurement of dietary fibre components: the importance of enzyme purity, activity and specificity.
McCleary, B. V. (2001), “Advanced Dietary Fibre Technology”, (B. V. McCleary and L. Prosky, Eds.), Blackwell Science, Oxford, U.K., pp. 89-105.

Dietary fiber and available carbohydrates.
McCleary, B. V. & Rossiter, P. C. (2007). “Dietary Fiber: An International Perspective for Harmonization of Health Benefits and Energy Values”, (Dennis T. Gordon and Toshinao Goda, Eds.), AACC International, Inc., pp. 31-59.

Measuring dietary fibre.
McCleary, B. V. (1999). The World of Ingredients, 50-53.

Two issues in dietary fiber measurement.
McCleary, B. V. (2001). Cereal Foods World, 46(4), 164-165.

Dietary fibre analysis.
McCleary, B. V. (2003). Proceedings of the Nutrition Society, 62, 3-9.

Measurement of novel dietary fibres.
McCleary, B. V. & Rossiter, P. (2004). Journal of AOAC International, 87(3), 707-717.

An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates.
McCleary, B. V. (2007). Analytical and Bioanalytical Chemistry, 389(1), 291-308.

Development and evaluation of an integrated method for the measurement of total dietary fibre.
McCleary, B. V., Mills, C. & Draga, A. (2009). Quality Assurance and Safety of Crops & Foods, 1(4), 213–224.

Determination of total dietary fiber (CODEX definition) by enzymatic-gravimetric method and liquid chromatography: collaborative study.
McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, L., Mugford, D. C., Champ, M. & Okuma, K. (2010). Journal of AOAC International, 93(1), 221-233.

Determination of insoluble, soluble, and total dietary fiber (codex definition) by enzymatic-gravimetric method and liquid chromatography: Collaborative Study.
McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, L., Mugford, D. C., Champ, M. & Okuma, K. (2012). Journal of AOAC International, 95(3), 824-844.

Measurement of total dietary fiber using AOAC method 2009.01 (AACC International approved method 32- 45.01): Evaluation and updates.
McCleary, B. V., Sloane, N., Draga, A. & Lazewska, I. (2013). Cereal Chemistry, 90(4), 396-414.

使用手冊：

K-ACHDF使用手冊

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可吸收碳水化合物/膳食纖維檢測試劑盒 Available Carbohydrates K-ACHDF

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