氨[快速]檢測試劑盒 , Ammonia (Rapid) Assay Kit , 貨號:K-AMIAR
中文品名:氨[快速]檢測試劑盒
品名:Ammonia (Rapid) Assay Kit
規格: 96次(手工) / 960次 (微孔板) / 960次(自動分析儀)
應用:氨[快速]檢測試劑盒,用于包括葡萄汁和葡萄酒以及其它食品、飲料樣本中氨含量的快速檢測。 輔助因子更強的穩定性,溶解后的輔助因子在4°C下可保存超過1年。適合手工、微孔板和自動分析儀分析。
原理:
食品、飲料和其它食材中氨的紫外檢測方法
檢測方法:分光光度法 @340 nm
反應時間:~ 5分鐘
檢測限: 0.07 mg/L
應用案例:
葡萄汁、葡萄酒、水果汁、軟飲料、奶制品(例如牛奶)、營養食品、醬油、蛋及蛋制品、奶酪、肉、加工肉、海鮮、烘焙產品、化肥、醫藥、煙草、化妝品、水、凱氏定氮、紙張(和紙板)、水和其他材料 (例如:生物培養、樣品等)
方法識別:
基于此原理的檢測方法被MEBAK接受認可。
試劑盒組成:
Bottle 1: |
緩沖液(36 ml,pH 8),添加α-酮戊二酸和疊氮化鈉(0.02% w/v)作為防腐劑 |
|
4°C下穩定超過 2年 |
Bottle 2: |
NADPH |
|
-20°C穩定超過5年 |
Bottle 3: |
谷氨酸脫氫酶(2.2 mL) |
|
4°C穩定超過2年 |
Bottle 4: |
氨標準溶液(5 mL, 0.04 mg/mL) 溶于0.02% (w/v)疊氮鈉 |
|
4°C穩定超過2年 |
優點:
使用谷氨酸脫氫酶,反應異常迅速
提供穩定的酶懸浮液 價格具有競爭力
所有試劑制備后可以穩定保存超過2年
官網提供Mega-Calc™ 軟件工具用于一站式原始數據處理
包含標準品
輔助因子更強的穩定性
適合手工、微孔板和自動分析儀分析
參考文獻:
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Combined intracellular nitrate and NIT2 effects on storage carbohydrate metabolism in Chlamydomonas. Remacle, C., Eppe, G., Coosemans, N., Fernandez, E. & Vigeolas, H. (2014). Journal of Experimental Botany, 65(1), 23-33.
A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis. Barth, C., Gouzd, Z. A., Steele, H. P. & Imperio, R. M. (2010). Journal of Experimental Botany, 61(2), 379-394.
Proteomic phenotyping of Novosphingobium nitrogenifigens reveals a robust capacity for simultaneous nitrogen fixation, polyhydroxyalkanoate production, and resistance to reactive oxygen species. Smit, A. M., Strabala, T. J., Peng, L., Rawson, P., Lloyd-Jones, G. & Jordan, T. W. (2012). Applied and Environmental Microbiology, 78(14), 4802-4815.
No effects of gluten in patients with self-reported non-celiac gluten sensitivity after dietary reduction of fermentable, poorly absorbed, short-chain carbohydrates. Biesiekierski, J. R., Peters, S. L., Newnham, E. D., Rosella, O., Muir, J. G. & Gibson, P. R. (2013). Gastroenterology, 145(2), 320-328.
The interaction between high ammonia diet and bile duct ligation in developing rats: assessment by spatial memory and asymmetric dimethylarginine. Huang, L. T., Chen, C. C., Sheen, J. M., Chen, Y. J., Hsieh, C. S. & Tain, Y. L. (2010). International Journal of Developmental Neuroscience, 28(2), 169-174.
Dietary protein excess during neonatal life alters colonic Microbiota and mucosal response to inflammatory mediators later in life in female pigs. Boudry, G., Jamin, A., Chatelais, L., Gras-Le Guen, C., Michel, C. & Le Huërou-Luron, I. (2013). The Journal of Nutrition, 143(8), 1225-1232.
Effects of enzymatic modification of wheat protein on the formation of pyrazines and other volatile components in the Maillard reaction. Lee, S. E., Chung, H. & Kim, Y. S. (2012). Food Chemistry, 131(4), 1248-1254.
使用手冊:
相關產品:
L-Arginine/Urea/Ammonia Assay Kit
Primary Amino Nitrogen Assay Kit (PANOPA)
Urea/Ammonia Assay Kit (Rapid)
L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)
L-Glutamine/Ammonia Assay Kit (Rapid)
D-Fructose/D-Glucose Assay Kit
L-Malic Acid Assay Kit (Manual Format)
Total Sulfite Assay Kit
共有-條評論【我要評論】