| 訂購貨號 | 產品名稱及規格 |
| 13020 | Cyanine5 NHS ester, 1 mg |
| 23020 | Cyanine5 NHS ester, 5 mg |
| 43020 | Cyanine5 NHS ester, 25 mg |
| 53020 | Cyanine5 NHS ester, 50 mg |
| 63020 | Cyanine5 NHS ester, 100 mg |
在過去的幾年里，CY5熒光團已經成為生命科學研究和診斷領域流行的分子標記物。這些熒光素的較強發射光為紅光，在這個范圍大多數CCD檢測器具有檢測MAX靈敏度，而且是生物物質低背景區。染料的顏色是很強烈的，因此在凝膠電泳中低至1nmol的量都能被肉眼觀測到。CY5 NHS ester是用于標記多肽、蛋白和寡核苷酸的氨基基團的活性染料。在標記反應中該染料需要少量的有機共溶劑（如DMF，DMSO）（請參照我們的推薦手冊以獲得更多幫助信息）。對于可溶性蛋白、各種各樣的多肽和寡核苷酸而言，這種染料是較理想而且成本低廉的。該染料在有機溶劑對小分子物質的標記也是有效的。對于更多的精細目標物，如易降解蛋白，如果DMF或DMSO會對其有影響，可以考慮water-soluble Cy5 NHS ester（水溶性Cy5 NHS ester染料），因為它們不需要有機溶劑助溶，而且具有和熒光染料類似的性質。
CY5熒光團可以與很多的儀器如熒光顯微鏡、成像儀、掃描儀、熒光分析儀等相兼容。許多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯（activated esters）。
推薦手冊
氨基分子的NHS酯標記（請聯系西寶生物獲取）
Cy® is a trademark of GE Healthcare.
S" style="color: rgb(61, 61, 61);">General properties
| Appearance: | dark blue powder |
| Molecular weight: | 616.19 |
| Molecular formula: |
C36H42ClN3O4 |
| CAS number: | 1032678-42-4, 350686-88-3 |
| Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 646 |
| Extinction coefficient at excitation maximum, Lmol-1cm-1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
Product citations
- Wilkinson, N.; Metaxas, A.; Ruud, E.; Raethke, E.; Wickramaratne, S.; Reineke, T.M.; Dutcher, C.S. Internal structure visualization of polymer – clay flocculants using fluorescence. Colloids and Interface Science Communications, 2016, 10–11, 1–5. doi:10.1016/j.colcom.2016.03.002
- Chen, K.; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327. doi: 10.1016/j.ijpharm.2016.05.060
- Wu, F.; Liu, C.; Chen, Y.; Yang, S.; Xu, J.; Huang, R.; Wang, X.; Li, M.; Liu, W.; Mao, W.; Zhou, X. Visualization of G-quadruplexes in gel and in live cells by a near-infrared fluorescent probe. Sensors and Actuators B: Chemical, 2016, 236, 268&?β?β牥戭硯;????鑈αndash;275. doi:10.1016/j.snb.2016.05.162
- Ray, J.; Shin, I.; Ilgu, M.; Bendickson, L.; Gupta, V.; Kraus, G.A.; Nilsen-Hamilton, M. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters. Methods in Enzymology, 2016, 572, 193–213. doi:10.1016/bs.mie.2016.02.028
- Hartley, J.M.; Zhang, R.; Gudheti, M.; Yang, J.; Kope?ek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59. doi: 10.1016/j.jconrel.2016.02.005
- Xu, X.; Li, L.; Zhou, Z.; Sun, W.; Huang, Y. Dual-pH responsive micelle platform for co-delivery of axitinib and doxorubicin.International Journal of Pharmaceutics, 2016, 507(1–2), 50–60. doi: 10.1016/j.ijpharm.2016.04.060
- Tran, T.-H.; Krishnan, S.; Amiji, M.M. MicroRNA-223 Induced Repolarization of Peritoneal Macrophages Using CD44 Targeting Hyaluronic Acid Nanoparticles for Anti-Inflammatory Effects. PLoS One, 2016, 11(5), e0152024. doi: 10.1371/journal.pone.0152024
- Liu, C.; Stonestrom, A.J.; Christian, T.; Yong, J.; Takase, R.; Hou, Y.-M.; Yang, X. Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction. Journal of Biological Chemistry, 2016, 291(19), 10426–10436. doi: 10.1074/jbc.M115.697789
- Li, L.; Sun, W.; Zhang, Z.; Huang, Y. Time-staggered delivery of docetaxel and H1S6A,F8A peptide for sequential dual-strike chemotherapy through tumor priming and nuclear targeting. Journal of Controlled Release, 2016, 232, 62–74. doi:10.1016/j.jconrel.2016.04.021
- Leenders, J.; Baker, M.B.; Pijpers, I.; Lafleur, R.; Albertazzi, L.; Palmans, A.R.A.; Meijer, E.W. Supramolecular polymerisation in water; elucidating the role of hydrophobic and hydrogen-bond interactions. Soft Matter, 2016, 12, 2887–2893. doi:10.1039/c5sm02843d
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